The first is the quality assessment of the produced protein preparations.
The major obstacle turned out to be poor _expression_ and low solubility. Therefore, screening for soluble _expression_ on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. Overall, HTP _expression_ screening in E.
Co-_expression_ of subunits within hosts such as Escherichia coli and insect cells has become more and more amenable, even at the level of high-throughput projects. Although important biologically, for the structural biologist disordered regions of proteins can be disastrous even preventing successful structure determination. Reports on new protein structures are particularly encouraged, as are papers on crystallographic binding studies, structural analysis of mutants and other structure-function studies.
Reports on new protein structures are particularly encouraged, as are papers on crystallographic binding studies, structural analysis of mutants and other structure-function studies. SPINE was able to bring together developers of software with users from the partner laboratories. coli and insect cells. The system is suitable for use in conventional laboratories or can be implemented in a medium- or high-throughput pipeline. Data processing and analysis, use of experimental phases and model construction were also addressed, albeit at a lower level.
Data processing and analysis, use of experimental phases and model construction were also addressed, albeit at a lower level.
coli and a database system dedicated to handle co-_expression_ data are described.
Reports of all actions taken are available for inspection via database modules and web services. Of the eight soluble proteins, five were crystallized using robotic nanolitre-drop crystallization trials, which led to four solved structures. coli and insect cells.
Overall, HTP _expression_ screening in E. The first is the quality assessment of the produced protein preparations.
Suitable vectors for HTP cloning are described and results from their use in _expression_ screening and protein-production pipelines are reported.
The success rate of protein _expression_ and structure solution is at least in keeping with that achieved in structural genomics programs.
Results are also reported from various case studies investigating different methods for performing co-_expression_ in E. coli and insect cells.
The major obstacle turned out to be poor _expression_ and low solubility.
Papers on crystallographic methods should be oriented towards biological crystallography, and may include new approaches to any aspect of structure determination or analysis.
Of the eight soluble proteins, five were crystallized using robotic nanolitre-drop crystallization trials, which led to four solved structures.
coli and a database system dedicated to handle co-_expression_ data are described. Therefore, screening for soluble _expression_ on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis.